diff --git a/nixos/tests/all-tests.nix b/nixos/tests/all-tests.nix
index 8ac3f6043d0..3745a0194b9 100644
--- a/nixos/tests/all-tests.nix
+++ b/nixos/tests/all-tests.nix
@@ -392,6 +392,7 @@ in
   proxy = handleTest ./proxy.nix {};
   prowlarr = handleTest ./prowlarr.nix {};
   pt2-clone = handleTest ./pt2-clone.nix {};
+  pulseaudio = discoverTests (import ./pulseaudio.nix);
   qboot = handleTestOn ["x86_64-linux" "i686-linux"] ./qboot.nix {};
   quorum = handleTest ./quorum.nix {};
   rabbitmq = handleTest ./rabbitmq.nix {};
diff --git a/nixos/tests/pulseaudio.nix b/nixos/tests/pulseaudio.nix
new file mode 100644
index 00000000000..4e2ce679acd
--- /dev/null
+++ b/nixos/tests/pulseaudio.nix
@@ -0,0 +1,71 @@
+let
+  mkTest = { systemWide ? false }:
+    import ./make-test-python.nix ({ pkgs, lib, ... }:
+      let
+        testFile = pkgs.fetchurl {
+          url =
+            "https://file-examples-com.github.io/uploads/2017/11/file_example_MP3_700KB.mp3";
+          hash = "sha256-+iggJW8s0/LfA/okfXsB550/55Q0Sq3OoIzuBrzOPJQ=";
+        };
+
+        makeTestPlay = key:
+          { sox, alsa-utils }:
+          pkgs.writeScriptBin key ''
+            set -euxo pipefail
+            ${sox}/bin/play ${testFile}
+            ${sox}/bin/sox ${testFile} -t wav - | ${alsa-utils}/bin/aplay
+            touch /tmp/${key}_success
+          '';
+
+        testers = builtins.mapAttrs makeTestPlay {
+          testPlay = { inherit (pkgs) sox alsa-utils; };
+          testPlay32 = { inherit (pkgs.pkgsi686Linux) sox alsa-utils; };
+        };
+      in {
+        name = "pulseaudio${lib.optionalString systemWide "-systemWide"}";
+        meta = with pkgs.lib.maintainers; {
+          maintainers = [ synthetica ] ++ pkgs.pulseaudio.meta.maintainers;
+        };
+
+        machine = { ... }:
+
+          {
+            imports = [ ./common/wayland-cage.nix ];
+            hardware.pulseaudio = {
+              enable = true;
+              support32Bit = true;
+              inherit systemWide;
+            };
+
+            environment.systemPackages = [ testers.testPlay pkgs.pavucontrol ]
+              ++ lib.optional pkgs.stdenv.isx86_64 testers.testPlay32;
+          } // lib.optionalAttrs systemWide {
+            users.users.alice.extraGroups = [ "audio" ];
+            systemd.services.pulseaudio.wantedBy = [ "multi-user.target" ];
+          };
+
+        enableOCR = true;
+
+        testScript = { ... }: ''
+          machine.wait_until_succeeds("pgrep xterm")
+          machine.wait_for_text("alice@machine")
+
+          machine.send_chars("testPlay \n")
+          machine.wait_for_file("/tmp/testPlay_success")
+          ${lib.optionalString pkgs.stdenv.isx86_64 ''
+            machine.send_chars("testPlay32 \n")
+            machine.wait_for_file("/tmp/testPlay32_success")
+          ''}
+          machine.screenshot("testPlay")
+
+          # Pavucontrol only loads when Pulseaudio is running. If it isn't, the
+          # text "Playback" (one of the tabs) will never show.
+          machine.send_chars("pavucontrol\n")
+          machine.wait_for_text("Playback")
+          machine.screenshot("Pavucontrol")
+        '';
+      });
+in builtins.mapAttrs (key: val: mkTest val) {
+  user = { systemWide = false; };
+  system = { systemWide = true; };
+}