diff --git a/pkgs/development/tools/biodiff/default.nix b/pkgs/development/tools/biodiff/default.nix
new file mode 100644
index 00000000000..340c3b736ca
--- /dev/null
+++ b/pkgs/development/tools/biodiff/default.nix
@@ -0,0 +1,23 @@
+{ lib, fetchFromGitHub, rustPlatform }:
+
+rustPlatform.buildRustPackage rec {
+  pname = "biodiff";
+  version = "1.0.1";
+
+  src = fetchFromGitHub {
+    owner = "8051Enthusiast";
+    repo = "biodiff";
+    rev = "v${version}";
+    sha256 = "sha256-M1hwuIe5+quxcvFAacBkxQMiQyN6lhtWA6hEi5Buoho=";
+  };
+
+  cargoSha256 = "sha256-NIt4D2/T7Zl7rgksbQeVo6cNBt6cZkUGTJGztnp6SB0=";
+
+  meta = with lib; {
+    description = "Hex diff viewer using alignment algorithms from biology";
+    homepage = "https://github.com/8051Enthusiast/biodiff";
+    changelog = "https://github.com/8051Enthusiast/biodiff/blob/v${version}/CHANGELOG";
+    license = licenses.mit;
+    maintainers = with maintainers; [ newam ];
+  };
+}
diff --git a/pkgs/top-level/all-packages.nix b/pkgs/top-level/all-packages.nix
index 575ca2a4c02..f0f95464a3e 100644
--- a/pkgs/top-level/all-packages.nix
+++ b/pkgs/top-level/all-packages.nix
@@ -4419,6 +4419,8 @@ with pkgs;
 
   brotli = callPackage ../tools/compression/brotli { };
 
+  biodiff = callPackage ../development/tools/biodiff { };
+
   biosdevname = callPackage ../tools/networking/biosdevname { };
 
   bluetooth_battery = python3Packages.callPackage ../applications/misc/bluetooth_battery { };