diff --git a/nixos/tests/all-tests.nix b/nixos/tests/all-tests.nix
index 5f4aefad890..a64f7c5ccbc 100644
--- a/nixos/tests/all-tests.nix
+++ b/nixos/tests/all-tests.nix
@@ -577,6 +577,7 @@ in {
   openvscode-server = handleTest ./openvscode-server.nix {};
   orangefs = handleTest ./orangefs.nix {};
   os-prober = handleTestOn ["x86_64-linux"] ./os-prober.nix {};
+  osquery = handleTestOn ["x86_64-linux"] ./osquery.nix {};
   osrm-backend = handleTest ./osrm-backend.nix {};
   overlayfs = handleTest ./overlayfs.nix {};
   pacemaker = handleTest ./pacemaker.nix {};
diff --git a/nixos/tests/osquery.nix b/nixos/tests/osquery.nix
new file mode 100644
index 00000000000..dc55fa82f8d
--- /dev/null
+++ b/nixos/tests/osquery.nix
@@ -0,0 +1,56 @@
+import ./make-test-python.nix ({ lib, pkgs, ... }:
+
+with lib;
+
+let
+  config_refresh = "10";
+  nullvalue = "NULL";
+  utc = false;
+in
+{
+  name = "osquery";
+  meta = with maintainers; {
+    maintainers = [ znewman01 lewo ];
+  };
+
+  nodes.machine = { config, pkgs, ... }: {
+    services.osquery = {
+      enable = true;
+
+      settings.options = { inherit nullvalue utc; };
+      flags = {
+        inherit config_refresh;
+        nullvalue = "IGNORED";
+      };
+    };
+  };
+
+  testScript = { nodes, ... }:
+    let
+      cfg = nodes.machine.services.osquery;
+    in
+    ''
+      machine.start()
+      machine.wait_for_unit("osqueryd.service")
+
+      # Stop the osqueryd service so that we can use osqueryi to check information stored in the database.
+      machine.wait_until_succeeds("systemctl stop osqueryd.service")
+
+      # osqueryd was able to query information about the host.
+      machine.succeed("echo 'SELECT address FROM etc_hosts LIMIT 1;' | osqueryi | tee /dev/console | grep -q '127.0.0.1'")
+
+      # osquery binaries respect configuration from the Nix config option.
+      machine.succeed("echo 'SELECT value FROM osquery_flags WHERE name = \"utc\";' | osqueryi | tee /dev/console | grep -q ${boolToString utc}")
+
+      # osquery binaries respect configuration from the Nix flags option.
+      machine.succeed("echo 'SELECT value FROM osquery_flags WHERE name = \"config_refresh\";' | osqueryi | tee /dev/console | grep -q ${config_refresh}")
+
+      # Demonstrate that osquery binaries prefer configuration plugin options over CLI flags.
+      # https://osquery.readthedocs.io/en/latest/deployment/configuration/#options.
+      machine.succeed("echo 'SELECT value FROM osquery_flags WHERE name = \"nullvalue\";' | osqueryi | tee /dev/console | grep -q ${nullvalue}")
+
+      # Module creates directories for default database_path and pidfile flag values.
+      machine.succeed("test -d $(dirname ${cfg.flags.database_path})")
+      machine.succeed("test -d $(dirname ${cfg.flags.pidfile})")
+    '';
+})