diff --git a/nixos/tests/k3s/default.nix b/nixos/tests/k3s/default.nix
index e758ca162fa..07d93c41c7a 100644
--- a/nixos/tests/k3s/default.nix
+++ b/nixos/tests/k3s/default.nix
@@ -4,4 +4,6 @@
 {
   # Run a single node k3s cluster and verify a pod can run
   single-node = import ./single-node.nix { inherit system pkgs; };
+  # Run a multi-node k3s cluster and verify pod networking works across nodes
+  multi-node = import ./multi-node.nix { inherit system pkgs; };
 }
diff --git a/nixos/tests/k3s/multi-node.nix b/nixos/tests/k3s/multi-node.nix
new file mode 100644
index 00000000000..afb8c78f233
--- /dev/null
+++ b/nixos/tests/k3s/multi-node.nix
@@ -0,0 +1,137 @@
+import ../make-test-python.nix ({ pkgs, ... }:
+  let
+    imageEnv = pkgs.buildEnv {
+      name = "k3s-pause-image-env";
+      paths = with pkgs; [ tini bashInteractive coreutils socat ];
+    };
+    pauseImage = pkgs.dockerTools.streamLayeredImage {
+      name = "test.local/pause";
+      tag = "local";
+      contents = imageEnv;
+      config.Entrypoint = [ "/bin/tini" "--" "/bin/sleep" "inf" ];
+    };
+    # A daemonset that responds 'server' on port 8000
+    networkTestDaemonset = pkgs.writeText "test.yml" ''
+      apiVersion: apps/v1
+      kind: DaemonSet
+      metadata:
+        name: test
+        labels:
+          name: test
+      spec:
+        selector:
+          matchLabels:
+            name: test
+        template:
+          metadata:
+            labels:
+              name: test
+          spec:
+            containers:
+            - name: test
+              image: test.local/pause:local
+              imagePullPolicy: Never
+              resources:
+                limits:
+                  memory: 20Mi
+              command: ["socat", "TCP4-LISTEN:8000,fork", "EXEC:echo server"]
+    '';
+    tokenFile = pkgs.writeText "token" "p@s$w0rd";
+  in
+  {
+    name = "k3s-multi-node";
+
+    nodes = {
+      server = { pkgs, ... }: {
+        environment.systemPackages = with pkgs; [ gzip jq ];
+        # k3s uses enough resources the default vm fails.
+        virtualisation.memorySize = 1536;
+        virtualisation.diskSize = 4096;
+
+        services.k3s = {
+          inherit tokenFile;
+          enable = true;
+          role = "server";
+          package = pkgs.k3s;
+          extraFlags = "--no-deploy coredns,servicelb,traefik,local-storage,metrics-server --pause-image test.local/pause:local --node-ip 192.168.1.1";
+        };
+        networking.firewall.allowedTCPPorts = [ 6443 ];
+        networking.firewall.allowedUDPPorts = [ 8472 ];
+        networking.firewall.trustedInterfaces = [ "flannel.1" ];
+        networking.useDHCP = false;
+        networking.defaultGateway = "192.168.1.1";
+        networking.interfaces.eth1.ipv4.addresses = pkgs.lib.mkForce [
+          { address = "192.168.1.1"; prefixLength = 24; }
+        ];
+      };
+
+      agent = { pkgs, ... }: {
+        virtualisation.memorySize = 1024;
+        virtualisation.diskSize = 2048;
+        services.k3s = {
+          inherit tokenFile;
+          enable = true;
+          role = "agent";
+          serverAddr = "https://192.168.1.1:6443";
+          extraFlags = "--pause-image test.local/pause:local --node-ip 192.168.1.2";
+        };
+        networking.firewall.allowedTCPPorts = [ 6443 ];
+        networking.firewall.allowedUDPPorts = [ 8472 ];
+        networking.firewall.trustedInterfaces = [ "flannel.1" ];
+        networking.useDHCP = false;
+        networking.defaultGateway = "192.168.1.2";
+        networking.interfaces.eth1.ipv4.addresses = pkgs.lib.mkForce [
+          { address = "192.168.1.2"; prefixLength = 24; }
+        ];
+      };
+    };
+
+    meta = with pkgs.lib.maintainers; {
+      maintainers = [ euank ];
+    };
+
+    testScript = ''
+      start_all()
+      machines = [server, agent]
+      for m in machines:
+          m.wait_for_unit("k3s")
+
+      # wait for the agent to show up
+      server.wait_until_succeeds("k3s kubectl get node agent")
+
+      for m in machines:
+          m.succeed("k3s check-config")
+          m.succeed(
+              "${pauseImage} | k3s ctr image import -"
+          )
+
+      server.succeed("k3s kubectl cluster-info")
+      # Also wait for our service account to show up; it takes a sec
+      server.wait_until_succeeds("k3s kubectl get serviceaccount default")
+
+      # Now create a pod on each node via a daemonset and verify they can talk to each other.
+      server.succeed("k3s kubectl apply -f ${networkTestDaemonset}")
+      server.wait_until_succeeds(f'[ "$(k3s kubectl get ds test -o json | jq .status.numberReady)" -eq {len(machines)} ]')
+
+      # Get pod IPs
+      pods = server.succeed("k3s kubectl get po -o json | jq '.items[].metadata.name' -r").splitlines()
+      pod_ips = [server.succeed(f"k3s kubectl get po {name} -o json | jq '.status.podIP' -cr").strip() for name in pods]
+
+      # Verify each server can ping each pod ip
+      for pod_ip in pod_ips:
+          server.succeed(f"ping -c 1 {pod_ip}")
+          agent.succeed(f"ping -c 1 {pod_ip}")
+
+      # Verify the pods can talk to each other
+      resp = server.wait_until_succeeds(f"k3s kubectl exec {pods[0]} -- socat TCP:{pod_ips[1]}:8000 -")
+      assert resp.strip() == "server"
+      resp = server.wait_until_succeeds(f"k3s kubectl exec {pods[1]} -- socat TCP:{pod_ips[0]}:8000 -")
+      assert resp.strip() == "server"
+
+      # Cleanup
+      server.succeed("k3s kubectl delete -f ${networkTestDaemonset}")
+
+      for m in machines:
+          m.shutdown()
+    '';
+  })